This kit allows for the determination of PTH concentrations in human serum,cell culture
supernates and other biological flids.The kit assay human PTH level in the sample,use
purified human PTH antibody to coat microtiter plate wells,make soild-phase
antibody,then add PTH to wells,Combined PTH antibody which with HRP labeled goat
anti-human become antibody-antigen-enzyme-antibody complex,add TMb substrate
solution,Tmb substrate becomes biue color at HRP enzyme-catalyzed,reaction is
terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450nm.the concentration of human PTH in the
sample is then determined by comparing the O.D.of the sample to the standard curve.